Sequence analysis of VP1, VP2 and VP3 genes of Infectious Bursal Disease Virus from a field outbreak in Kerala, India

Infectious bursal disease (IBD), caused by infectious virus (IBDV), is one among the top five diseases of poultry that discernibly affects commercial industry. The mutating viral genome IBDV accounts for outbreaks in fields even after following stringent biosecurity measures and vaccination protocols. present study focussed on characterisation an field virus, IBD/CVAS/6, from a vaccinated flock Kerala, based sequence analysis VP1, VP2 VP3 genes. Bursa Fabricius samples collected 26 days-old chicken suspected outbreak Thiruvananthapuram district Kerala formed subject study. Reverse transcriptase-polymerase chain reaction (RT-PCR) targeting gene confirmed presence sample. revealed deduced amino acid VP1 IBD/CVAS/6 was 100 per cent homologous with attenuated very virulent vaccine strain Israel, mb Ventri plus India. also similarity strains except single variation S745N its sequence. phylogenetic it’s close relation mb, ks. characteristic marker motifs ‘SWSASGS’ ‘TDN’ were genes, respectively. Hence, obtained has emerged hence report involvement intermediate Kerala. role virulence cannot be accounted study, however might related to or variations other genes due inefficiency protocol followed, which can defined only further studies. first focussing IBDV.